Fig. 6

FBA of ΔpfkA (JW3887) and WH04. The FBA was constrained by the ¹³C-flux ratio and optimized for biomass accumulation. a Heat Map of the difference (Flux_mutant-Flux_WT) in the optimal fluxes (normalized to total carbon uptake rate, which was set to one) between mutant strains (ΔpfkA-JW3887 and WH04) and WT (green showed increased relative flux compared to wild type, red showed decreased relative flux compared to wild type). b Absolute total productions of ATP, NADH, and NADPH. c Simulated and measured growth rates of the different strains and growth conditions. The wild-type strain glucose uptake rate was assumed to be 8.5 mmol/h/g DCW