Table 1 Strains/plasmids used

Plasmids

 pBbE5c-YFP

Backbone vector expressing YFP

–

–

[48]

 pEDW

Derived from pBbE5c-YFP with gene replaced by edd and eda

–

–

This study

Strains

 BW25113

Keio collection WT(rrnB3 ∆lacZ4787 hsdR514 ∆araBAD567 ∆rhaBAD568 rph-1)

0.78 ± 0.02

–

[15]

 JW3887

BW25113 ∆pfkA

0.18 ± 0.02

–

 WH01

BW25113 (pBbE5c-YFP)

0.56 ± 0.07

JBEI-14575

This study

 WH02

JW3887 (pBbE5c-YFP)

0.13 ± 0.01

JBEI-14585

This study

 WH03

BW25113 (pEDW)

0.49 ± 0.02

JBEI-11465

This study

 WH04

JW3887 (pEDW)

0.18 ± 0.01

JBEI-11468

This study