Table 1 Overview of AHPD-experiments

0-0

Inoculum

A

0-1

Till

P1) Sodium adaptation

Autogenerated

−110

0.10

2.0

–

NaAc

–

6.4

–

B

till

0-10

−14

0.10

2.9

NaAc

6.4

H

I-1

P2) Glucose and pressure adaptation

Autogenerated

0–7

0.10

2.9

Glucose

7.2

I

I-2

7–14

0.27

Glucose

7.2

I-3

14–21

0.60

4.0

Glucose

7.2

J

I-4

21–56

0.10

Glucose

14.4

I-5

56–63

0.65

3.8

Glucose

7.2

K

I-6

P3) High pressure operation- A

Autogenerated

63–70

0.10

4.0

Glucose

14.4

L

I-7

70–77

0.60

4.7

Glucose

14.4

N

I-8

77–84

0.90

5.5

Glucose

14.4

O

I-9

84–93

1.22

6.3

Glucose

14.5

P

I-10

93–107

1.68

7.1

Glucose

14.4

Q

I-11

P4) High pressure operation- B

Autogenerated

107–114

0.10

2.0

Glucose

14.4

R

I-12

114–128

0.62

4.0

Glucose

14.4

S

I-13

128–135

0.88

5.0

Glucose

7.2

T

I-14^b

135–149

1.06

3.6

gluc + HAc

14.4

U

I-15^b

P5) Reactor Recovery

Autogenerated

149–157

0.10

gluc + HAc

7.2

V

I-16^b

pH₂

157–169

0.10

HAc + H₂

3.6 + 0.1^c

I-17

P6) High pressure operation- C

Autogenerated

169–176

0.10

Glucose

7.2

I-18

176–183

0.35

Glucose

7.2

W

I-19

183–192

0.64

Glucose

14.4

X

II-1

Stimulation Propionate degradation in eight-litre reactor

Autogenerated

248–257

0.10

Glucose

14.4

II-2

pH₂

257–261

0.30

H₂

0.27^c

II-3

pH₂

261–268

0.36

H₂

0.40^c

III-1

Kinetics Propionate degradation in 0.6-litre reactors

pCO₂

268–290

0.00

2.2

Propionate

0.37

III-2

0.10

2.2

Propionate

0.37

Y

III-3

0.30

2.2

Propionate

0.37

III-4

0.50

2.2

Propionate

0.37

IV-1

pH-effect Propionate degradation pH 8.0

pN₂

290–297

0.10

1.0

Propionate

1.8

IV-2

pCO₂

0.05

1.0

Propionate

1.8

IV-3

pH-effect Propionate degradation pH 6.3

pN₂

0.10

1.0

Propionate

1.8

Z

IV-4

pCO₂

<0.60

1.0

Propionate

1.8