Fig. 2
From: Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
Design and screening of tetracycline-inducible promoter variants with catP as a reporter. Shown in a are the sequences of the original Tet3n0 promoter and seven synthetic inducible promoters. The randomized non-conserved bases in −35 and −10 promoter elements are highlighted in red. Shown in b is the activity of catP reporter under tet3no and inducible promoters 1–12. The catP activity is expressed in Units/g protein. The inset shows the catP activity of weak promoters on a smaller scale