Fig. 3
From: Comparison of aldehyde-producing activities of cyanobacterial acyl-(acyl carrier protein) reductases
Activity and substrate specificity of eight representative AARs. a The amounts of hydrocarbons produced in E. coli coexpressing AAR and 73102 ADO. The amounts of pentadecane (C15:0), heptadecene (C17:1), and heptadecane (C17:0) and their combined total amount are shown in red, green, blue, and gray, respectively. The values are normalized to the total amount of hydrocarbons produced in E. coli coexpressing 7942 AAR and 73102 ADO. b The aldehyde-producing activity of AAR relative to that of 7942 AAR. The values are expressed as the amount of hydrocarbons normalized to the amount of the soluble form of AAR. c Fractions of pentadecane, heptadecene, and heptadecane relative to the total amount of hydrocarbons produced in E. coli, indicating the substrate specificity of AAR. d The substrate specificity of AAR relative to that of 7942 AAR. The vertical axis for pentadecane and heptadecene is shown on the left side, and that for heptadecane is shown on the right side. In all panels, the abscissa shows the names of host strains of AARs, and the right graph indicates the results for 7942 AAR and 9313 AAR with plasmids containing a mutant T7 promoter; in such cases, the expression level of AAR was decreased