Fig. 5

Structure and identification strategy of the assembled and integrated ten-gene pathway. a Structure and identification strategy of the assembled and integrated ten-gene pathway in YORWΔ17 site. b PCR gel picture of identification PCRs for assembly and integration. F1 to F7 indicate seven PCRs designed to confirm correct assembly and integration, 1 to 11 represent 11 strains picked from SC-His plate. Strains 4, 9, and 11 were proved to have all PCR fragments as illustrated in the red boxes. c PCR gel picture for analyzing whether all ten genes were integrated in Strains 4, 9, and 11. G1 to G10 indicate PCRs for gene identification. Control denotes the control gene ACT1. Negative control had no PCR bands and is not included here. d HPLC analysis for target product of the ten-gene biosynthetic pathway