Fig. 6

FA composition of C. gracilis cells grown in various culture media. Cells grown for 10 days in various culture media, including media with low salinity; FA composition assessed by GC–MS, colored box identities on right; each FA as proportion of total FA; and mean values plotted (n = 3). The following media used F/2-enriched seawater with vitamins or without vitamins (1 and 2, respectively); F/2-enriched seawater with 1, 2, or 3% sea salts (3–5, respectively); F/2-enriched NaCl medium with 0, 0.5, 1, 2, 3, or 4% NaCl (6–11, respectively); F/2-enriched 1% NaCl medium with urea (12); Daigo IMK medium (13); Daigo IMK medium with urea (14); seawater diluted with one or two volumes of sewage water (15 and 16, respectively); 2% liquid fertilizer supernatant in seawater (17); 2, 1, or 0.5% liquid fertilizer (18–20, respectively) in seawater; and vitamins added to media 1, 13, and 14