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Table 3 Effects of salinity on growth and TAG content of two diatoms, C. gracilis and P. tricornutum

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

 

Concentration of NaCl (%)

0

0.5

1

2

3

4

C. gracilis

 Doubling time (h)

11.1 ± 0.5

9.52 ± 0.48

9.16 ± 0.17

9.75 ± 0.20

11.3 ± 1.5

15.7 ± 0.4

 Cell density on 4th day (106 cells/mL)

2.41 ± 0.19

4.41 ± 0.67

5.32 ± 0.14

5.24 ± 0.26

3.64 ± 0.80

2.24 ± 0.81

 Cell density on 10th day (106 cells/mL)a

3.89 ± 0.45

5.30 ± 0.15

6.29 ± 0.31

6.47 ± 0.43

3.63 ± 0.32

2.71 ± 0.23

 Volume-specific TAG on 10th day (mg/L)

223 ± 18

234 ± 43

272 ± 4

223 ± 6

172 ± 3

69.3 ± 28.6

 Cell-specific TAG on 10th day (pg/cell)a

73.1 ± 7.3

46.2 ± 4.9

47.0 ± 2.5

35.5 ± 3.9

25.5 ± 0.6

19.3 ± 5.0

P. tricornutum

 Doubling time (h)

n.d.

n.d.

14.6 ± 0.2

15.8 ± 0.4

15.4 ± 0.7

19.0 ± 0.9

 Cell density on 4th day (106 cells/mL)

n.d.

n.d.

14.9 ± 0.4

15.4 ± 0.2

13.2 ± 0.6

11.6 ± 0.3

 Cell density on 10th day (106 cells/mL)

n.d.

n.d.

16.4 ± 1.0

16.6 ± 9.2

14.9 ± 0.1

13.8 ± 0.6

 Volume-specific TAG on 10th day (mg/L)

n.d.

n.d.

183 ± 11

180 ± 9

158 ± 14

161 ± 15

 Cell-specific TAG on 10th day (pg/cell)

n.d.

n.d.

11.2 ± 0.4

10.9 ± 1.1

10.6 ± 0.8

11.7 ± 0.6

  1. Cells cultivated in media containing various NaCl concentrations; media enriched with F/2 without vitamins and MgSO4, CaCl2, KCl, and NaHCO3 added, as described in “Methods” section
  2. n.d. not determined; n = 3–4
  3. a n = 2