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Table 4 Effects of urea on growth and TAG content of C. gracilis

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

 

Seawater

Marine Art

1% NaCl-based media

F/2

Urea-F/2

F/2 + 70 ppm urea

IMK

IMK + 70 ppm urea

F/2

Urea-F/2

F/2 + 70 ppm urea

Doubling time (h)

10.3 ± 0.1

8.93 ± 0.10

10.5 ± 0.1

10.2 ± 0.4

8.61 ± 0.25

9.16 ± 0.17

9.28 ± 0.16

9.63 ± 0.40

Cell density on 4th day (106 cells/mL)

4.72 ± 0.05

6.01 ± 0.16

6.36 ± 0.06

5.22 ± 0.22

5.67 ± 0.02

5.37 ± 0.37

5.36 ± 0.13

5.52 ± 0.10

Cell density on 10th day (106 cells/mL)

6.64 ± 0.01

7.04 ± 0.02

8.31 ± 0.18

6.73 ± 0.14

6.19 ± 0.05

6.59 ± 0.34

5.51 ± 0.22

7.08 ± 0.13

Volume-specific TAG on 10th day (mg/L)

163 ± 3

179 ± 9

150 ± 6

87.6 ± 5.3

76.6 ± 5.0

272 ± 4

260 ± 6

159 ± 15

Cell-specific TAG on 10th day (pg/cell)

24.1 ± 0.8

26.7 ± 2.6

18.0 ± 0.5

13.8 ± 1.1

11.9 ± 0.9

25.7 ± 1.9

47.4 ± 2.4

21.1 ± 2.1

  1. Cells cultivated in media enriched with F/2 without vitamins; urea-F/2 medium contained urea not nitrate at same molar concentration; F/2 + 70 ppm urea and IMK + 70 ppm urea media contained urea in addition to nitrate; 1% NaCl-based medium in place of seawater contained 1% NaCl and MgSO4, CaCl2, KCl, and NaHCO3 added, as described in “Methods” section; and n = 3