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Table 7 Use of liquid fertilizer as the nutrient source in culture media

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

  2% supernatant of liquid fertilizer 0.5% liquid fertilizer 1% liquid fertilizer 2% liquid fertilizer
1st subculture 2nd subculture 1st subculture 2nd subculture 1st subculture 2nd subculture 1st subculture 2nd subculture
Doubling time (h) 17.6 ± 0.5 12.5 ± 0.3 10.7 ± 0.1 14.5 ± 0.1 11.8 ± 0.1 11.6 ± 0.3 15.9 ± 0.7 15.8 ± 0.5
Cell density on 4th day (106 cells/mL) 3.69 ± 0.06 4.87 ± 0.10 4.73 ± 0.12 4.61 ± 0.06 5.19 ± 0.08 6.65 ± 0.16 6.12 ± 0.09 6.88 ± 0.11
Cell density on 10th day (106 cells/mL) 6.06 ± 0.37 6.69 ± 0.27 5.38 ± 0.05 5.59 ± 0.10 7.71 ± 0.01 8.75 ± 0.08 11.6 ± 0.1 9.88 ± 0.05
TAG on 10th day (mg/L) 220 ± 5 181 ± 3 94.9 ± 3.4 93.1 ± 3.8 165 ± 4 217 ± 15 213 ± 2 270 ± 15
  1. Liquid fertilizer diluted tenfold (10% by vol) with pure water; resulting 10% solution, with or without removing suspended materials, diluted further to 5, 10, and 20% (v/v) with seawater to obtain indicated amounts of liquid fertilizer culture media; cells cultured in resulting medium; cells used to inoculate liquid fertilizer medium cultivated in F/2-enriched seawater (1st subculture) or subcultured once in same liquid fertilizer medium (2nd subculture); and n = 3