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Table 1 Summary of the dissociation constants and stoichiometry obtained by isothermal titration calorimetry for the binding of chitooligosaccharides to the SusD-like proteins

From: A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitin

Protein Substrate KD (µM) n
CusDI GlcNAc 1470 ± 210 1a
Chitobiose 25.6 ± 2.3 1.21 ± 0.07
Chitotriose 8.51 ± 0.51 0.88 ± 0.08
Chitotetraoseb 38.3 ± 4.3 1.19 ± 0.03
Chitopentaose 12.8 ± 0.21 0.79 ± 0.02
Chitohexaose 16.1 ± 0.42 0.98 ± 0.06
CusDII Chitopentaose 236 ± 8 1.65 ± 0.07
Chitohexaose 41.2 ± 1.0 1.50 ± 0.29
  1. Error margins represent standard errors of the mean from 2–3 titration experiments unless otherwise noted
  2. a n value was constrained to 1 in a one-site binding model; see “Methods” section
  3. bSingle titration performed; errors are displayed for the fitting to a one-site binding model
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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