Biotechnology for Biofuels and Bioproducts

Table 1 Summary of the dissociation constants and stoichiometry obtained by isothermal titration calorimetry for the binding of chitooligosaccharides to the SusD-like proteins

From: A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitin

Protein	Substrate	K_D (µM)	n
CusD_I	GlcNAc	1470 ± 210	1^a
	Chitobiose	25.6 ± 2.3	1.21 ± 0.07
	Chitotriose	8.51 ± 0.51	0.88 ± 0.08
	Chitotetraose^b	38.3 ± 4.3	1.19 ± 0.03
	Chitopentaose	12.8 ± 0.21	0.79 ± 0.02
	Chitohexaose	16.1 ± 0.42	0.98 ± 0.06
CusD_II	Chitopentaose	236 ± 8	1.65 ± 0.07
CusD_II	Chitohexaose	41.2 ± 1.0	1.50 ± 0.29

Error margins represent standard errors of the mean from 2–3 titration experiments unless otherwise noted
^a n value was constrained to 1 in a one-site binding model; see “Methods” section
^bSingle titration performed; errors are displayed for the fitting to a one-site binding model

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