Fig. 8From: Succinate production positively correlates with the affinity of the global transcription factor Cra for its effector FBP in Escherichia coli The combination of Cra and acep by EMSA and ITC experiments. EMSA experiment with increasing concentrations of pure Cra protein. Molar ratios of acep:Cra were 1:0 (lane 1), 1:0.5 (lane 2), 1:1 (lane 3), 1:2 (lane 4), 1:4 (lane 5), 1:8 (lane 6), and 1:10 (lane 7). acep (1.5 μM) in the absence of FBP (a) and the gray value determined using Quantity one (b). acep (1.5 μM) in the presence of 1 mM FBP (c) and the gray value (d). ITC experiment with 15 mM wild-type Cra or mutations binding to 1 mM acep DNA with 50 mM FBP (e)Back to article page