Table 4 Bacterial strains used in this study
Strain | Genotype | Reference/source |
|---|---|---|
E. coli Top 10 | F-mcrA ∆ (mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆ (ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
NCIMB 11955 | TMO | |
DSM 2542 | DSM | |
TM242 | ldh − pfl − pdh up | TMO |
DSMZ 7263 | DSM | |
DSMZ 5366 | DSM | |
11955ΔpyrE | ΔpyrE | This study |
LS001 | ΔpyrEΔldh | This study |
LS003 | ΔpyrEΔldh pdh up | This study |
LS004 | ΔpyrEΔldh Δpfl pdh up | This study |
LS005 | ΔpyrEΔldh pfl − pdh up | This study |
LS242 | Δldh Δpfl pdh up | This study |
TM89 | ldh − | TMO |
TM89ΔpyrE | ΔpyrE ldh − | This study |
TM003 | ΔpyrE ldh − pdh up | This study |
TM004 | ΔpyrE ldh − pdhp u Δpfl | This study |
TM005 | ΔpyrE ldh − pdh up pfl − | This study |
LS006 | ΔpyrE ldh − pdh up pfl − Δaprt | This study |