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Table 4 Bacterial strains used in this study

From: Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955

Strain Genotype Reference/source
E. coli Top 10 F-mcrA ∆ (mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆ (ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
NCIMB 11955   TMO
DSM 2542   DSM
TM242 ldh pfl pdh up TMO
DSMZ 7263   DSM
DSMZ 5366   DSM
11955ΔpyrE ΔpyrE This study
LS001 ΔpyrEΔldh This study
LS003 ΔpyrEΔldh pdh up This study
LS004 ΔpyrEΔldh Δpfl pdh up This study
LS005 ΔpyrEΔldh pfl pdh up This study
LS242 Δldh Δpfl pdh up This study
TM89 ldh TMO
TM89ΔpyrE ΔpyrE ldh This study
TM003 ΔpyrE ldh pdh up This study
TM004 ΔpyrE ldh pdhp u Δpfl This study
TM005 ΔpyrE ldh pdh up pfl This study
LS006 ΔpyrE ldh pdh up pfl Δaprt This study