Fig. 1

Growth performance of the evolved CEN.PK113 derivatives JL1, PW-1, and PW-2, and the wild-type strain CBS 6412-13A in synthetic glycerol medium. The unevolved strains CEN.PK113-1A and CEN.PK113-7D showing no growth on glycerol at all were included in the experiment as negative controls. All evolved strains were subjected to five serial transfers in synthetic glucose medium before quantifying the growth in synthetic glycerol medium. Data from one representative experiment out of three independent biological replicates are shown. Average values and standard deviations of µ_max are shown in the box