Fig. 2

Experimental design and in-culture substrate saccharification. a A. niger was cultured with the indicated untreated and pretreated substrates for the times indicated (n = 3). For the cultures with the HT-pretreated straw, it was not possible to obtain sufficient RNA for RNA-seq from the cultures from the later time points. b Measurements of xylan in the initial substrates and residual xylan from the solids recovered from the fungal cultures after 24 h and 5 d (where available). Data are given as the mean (n = 3) of biological replicate flasks with error bars representing standard errors, or for the initial substrates as the mean with standard errors (n = 2) of technical hydrolysis replicates. c Monosaccharide profiles in the fungal culture filtrates over time. The error bars represent standard errors (n = 3). AC only = autoclaved substrate only