Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

Table 5 Summary of enzyme specificities detected by glycome profiling or oxime-NIMS

Enzyme	GH family	Substrates identified^a
CMX00_3a	GH5	Oat spelt xylan (β-1,4-xylose)^b
		Tamarind seed xyloglucan^c
		Cellulose (β-1,4-glucose)
		NON-FUC XG epitopes (structures not known)
		FUC-XG epitopes (structures not known)
		XYLAN-3 epitopes (structures not known)
		XYLAN-6 epitopes (unsubstituted xylan)^d
		XYLAN-7 epitopes (unsubstituted xylan)^e
		CCRC-M160
		CCRC-M137
XynY	GH10	Oat spelt xylan (β-1,4-xylose)^b
		Wheat arabinoxylan^f
		XYLAN-3 epitopes, structures not known
		XYLAN-4 epitopes
		CCRC-M154, arabinosylated-xylan
		XYLAN-5 epitopes, methyl GlcA-substituted xylan
		XYLAN-6 epitopes (unsubstituted xylan)⁴
		XYLAN-7 epitopes (unsubstituted xylan)⁵
		CCRC-M160
		CCRC-M137
		CCRC-M152
		CCRC-M149
XynA	GH11	Oat spelt xylan (β-1,4-xylose)^b
		Tamarind seed xyloglucan^c
		Wheat arabinoxylan^f
		XYLAN-6 epitopes (unsubstituted xylan)^d
		XYLAN-7 epitopes (unsubstituted xylan)^e
		CCRC-M160
		CCRC-M137

^aReactions with purified hemicellulose polysaccharides detected by DNS; reaction with cellulose in AFEX-treated CS and SG detected by oxime-NIMS; reaction of hemicellulose fraction in CS and SG detected by glycome profiling and oxime-NIMS
^bβ-1,4-xylose with ~70:15:10 composition of xylose:glucose:arabinose
^cβ-1,4-glucose with α-1,6 xylose branching at on average, 3 out of 4 glucosyl backbone residues
^dStretches of unsubstituted β-1,4-xylan with at least four xylose units
^eStretches of unsubstituted β-1,4-xylan with at least three xylose units
^fβ-1,4-xylose with 38:62 composition of arabinose and xylose, less than 1% of glucose, galactose, and mannose

ISSN: 2731-3654