Skip to main content

Advertisement

Table 5 Summary of enzyme specificities detected by glycome profiling or oxime-NIMS

From: Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

Enzyme GH family Substrates identifieda
CMX00_3a GH5 Oat spelt xylan (β-1,4-xylose)b
Tamarind seed xyloglucanc
Cellulose (β-1,4-glucose)
NON-FUC XG epitopes (structures not known)
FUC-XG epitopes (structures not known)
XYLAN-3 epitopes (structures not known)
XYLAN-6 epitopes (unsubstituted xylan)d
XYLAN-7 epitopes (unsubstituted xylan)e
 CCRC-M160
 CCRC-M137
XynY GH10 Oat spelt xylan (β-1,4-xylose)b
Wheat arabinoxylanf
XYLAN-3 epitopes, structures not known
XYLAN-4 epitopes
 CCRC-M154, arabinosylated-xylan
XYLAN-5 epitopes, methyl GlcA-substituted xylan
XYLAN-6 epitopes (unsubstituted xylan)4
XYLAN-7 epitopes (unsubstituted xylan)5
 CCRC-M160
 CCRC-M137
 CCRC-M152
 CCRC-M149
XynA GH11 Oat spelt xylan (β-1,4-xylose)b
Tamarind seed xyloglucanc
Wheat arabinoxylanf
XYLAN-6 epitopes (unsubstituted xylan)d
XYLAN-7 epitopes (unsubstituted xylan)e
 CCRC-M160
 CCRC-M137
  1. aReactions with purified hemicellulose polysaccharides detected by DNS; reaction with cellulose in AFEX-treated CS and SG detected by oxime-NIMS; reaction of hemicellulose fraction in CS and SG detected by glycome profiling and oxime-NIMS
  2. bβ-1,4-xylose with ~70:15:10 composition of xylose:glucose:arabinose
  3. cβ-1,4-glucose with α-1,6 xylose branching at on average, 3 out of 4 glucosyl backbone residues
  4. dStretches of unsubstituted β-1,4-xylan with at least four xylose units
  5. eStretches of unsubstituted β-1,4-xylan with at least three xylose units
  6. fβ-1,4-xylose with 38:62 composition of arabinose and xylose, less than 1% of glucose, galactose, and mannose