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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: A versatile 2A peptide-based bicistronic protein expressing platform for the industrial cellulase producing fungus, Trichoderma reesei

Fig. 2

Screening of transformants for identification of Cel7A-expressing colonies. Individual transformant colonies arising from the two different plasmid constructs (cel7A-2A-eGFP and eGFP-2A-Cel7A) were transferred to liquid medium containing hygromycin and grown for 3 days at 30 °C. Western blotting of secretome was performed for a few of the selected colonies from the 24-well plates using anti-Cel7A antibody. Lanes. M molecular weight marker, A, B, C, and D followed by numbers represent well coordinates, C Cel7A control

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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