Fig. 5

Correlation between fluorescence intensity and Cel7A expression in the C2G orientation. Individual transformants were transferred to three 24-well plates containing MAG medium supplemented with hygromycin and allowed to grow for 3 days at 30 °C. Rows are labeled as A–D. Columns are labeled as 1–6. Plates are labeled as plate A, plate B, and plate C. a Mycelia-containing plates visualized under blue light. b The same plates were also visualized using FluorChem Q imaging system (Cell Biosciences, Santa Clara, CA, USA). c Western blotting on selected colonies using anti-Cel7A antibody. Each lane represents secretome obtained from one well of the 24-well plate. M marker, letters followed by numbers represent coordinates on the 24-well plates. The first letter represents the plate ID, the second letter represents the row, and numbers following the two letters represent the column number. Coordinates D4, D5, and D6 in each plate represent the control strains, AST1116 (Cel7A-deleted T. reesei QM6a strain), JLT102A (AST1116 expressing native Cel7A under the eno promoter), and SV004 (Cel7A-2A-eGFP cassette containing very high eGFP fluorescence strain; colony A5-4 in Fig. 3a)