Fig. 6

Correlation between fluorescence intensity and Cel7A expression in the G2C orientation. Individual transformants were transferred to three 24-well plates containing MAG medium supplemented with hygromycin and allowed to grow for 3 days at 30 °C. Rows are labeled as A–D. Columns are labeled as 1–6. Plates are labeled as plate A, plate B, and plate C. a Mycelia-containing plates visualized under blue light. b The same plates were also visualized using FluorChem Q imaging system (Cell Biosciences, Santa Clara, CA, USA). c Western blotting on selected colonies using anti-Cel7A antibody. Each lane represents secretome obtained from one well of the 24-well plate. M marker, Alphabets followed by numbers represent coordinates on the 24-well plates. The first alphabet represents the plate ID, the second alphabet represents the row, and numbers following the two alphabets represent the column number. Coordinates D4, D5, and D6 in each plate represent the control strains, AST1116 (Cel7A-deleted strain), JLT102A (Cel7A-expressing strain in a Cel7A null background), and SV004 (Cel7A-2A-eGFP cassette containing very high eGFP fluorescence strain; colony A5-4 in Fig. 3a)