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Table 1 Sequence of solvents used in the method of separation of total lipids and beta-carotene

From: Coupling and uncoupling of triglyceride and beta-carotene production by Dunaliella salina under nitrogen limitation and starvation

Spotting of the sample

0.5 mL of hexane, 1.6% of diethyl-ether

Fraction A

One column volume of hexane, 1.6% of diethyl-ether

Fraction B

One column volume of hexane, 1.6% of diethyl-ether

Fraction C

One column volume of hexane, 1.6% of diethyl-ether

Fraction D

Five column volume of chloroform/methanol (1:1)

Three column volume of methanol

  1. Each fraction was then analyzed by thin-layer chromatography to validate the method resolution
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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