Skip to main content

Advertisement

Table 1 Sequence of solvents used in the method of separation of total lipids and beta-carotene

From: Coupling and uncoupling of triglyceride and beta-carotene production by Dunaliella salina under nitrogen limitation and starvation

Spotting of the sample 0.5 mL of hexane, 1.6% of diethyl-ether
Fraction A One column volume of hexane, 1.6% of diethyl-ether
Fraction B One column volume of hexane, 1.6% of diethyl-ether
Fraction C One column volume of hexane, 1.6% of diethyl-ether
Fraction D Five column volume of chloroform/methanol (1:1) Three column volume of methanol
  1. Each fraction was then analyzed by thin-layer chromatography to validate the method resolution