Skip to main content

Table 2 Cell growth, glucose consumption, ethanol formation, and furan aldehydes conversion of recombinant Z. mobilis strains

From: Enhancement of furan aldehydes conversion in Zymomonas mobilis by elevating dehydrogenase activity and cofactor regeneration

Inhibitors

Recombinant strains

Cell growth (OD600)

Glucose consumption (g/L)

Ethanol production (g/L)

Inhibitor conversion (g/L)

Furfural

ZM4(pHW20a-gfp) (Control)

0.361 ± 0.003

4.81 ± 0.58

1.37 ± 0.02

1.13 ± 0.09

ZM4(pHW20a-ZMO0062)

0.387 ± 0.003

4.13 ± 0.26

1.36 ± 0.04

1.04 ± 0.03

ZM4(pHW20a-ZMO1696)

0.404 ± 0.000

4.28 ± 0.19

1.39 ± 0.02

1.18 ± 0.03

ZM4(pHW20a-ZMO1722)

0.393 ± 0.004

4.78 ± 0.22

1.56 ± 0.06

1.34 ± 0.06

ZM4(pHW20a-ZMO1771)

0.378 ± 0.027

4.60 ± 0.26

1.49 ± 0.00

1.51 ± 0.02

ZM4(pHW20a-ZMO1993)

0.359 ± 0.009

4.87 ± 0.03

1.44 ± 0.00

1.02 ± 0.02

ZM4(pHW20a-ZMO0976)

0.362 ± 0.007

4.09 ± 0.05

1.37 ± 0.06

0.99 ± 0.05

ZM4(pHW20a-ZMO1344)

0.380 ± 0.002

4.56 ± 0.04

1.58 ± 0.03

1.01 ± 0.03

ZM4(pHW20a-ZMO1673)

0.314 ± 0.008

3.71 ± 0.01

1.09 ± 0.20

0.93 ± 0.03

ZM4(pHW20a-ZMO1773)

0.293 ± 0.002

3.19 ± 0.24

1.17 ± 0.14

0.93 ± 0.16

ZM4(pHW20a-ZMO1984)

0.348 ± 0.009

4.80 ± 0.09

1.39 ± 0.09

1.00 ± 0.02

HMF

ZM4(pHW20a-gfp) (Control)

0.419 ± 0.005

6.47 ± 0.04

2.26 ± 0.14

1.40 ± 0.09

ZM4(pHW20a-ZMO0062)

0.439 ± 0.009

5.57 ± 0.25

2.12 ± 0.17

1.43 ± 0.02

ZM4(pHW20a-ZMO1696)

0.497 ± 0.014

6.48 ± 0.07

2.04 ± 0.13

1.67 ± 0.08

ZM4(pHW20a-ZMO1722)

0.427 ± 0.021

6.02 ± 0.04

1.89 ± 0.05

1.85 ± 0.03

ZM4(pHW20a-ZMO1771)

0.471 ± 0.001

5.96 ± 0.24

2.41 ± 0.01

1.89 ± 0.04

ZM4(pHW20a-ZMO1993)

0.419 ± 0.001

5.92 ± 0.42

2.16 ± 0.08

1.19 ± 0.08

ZM4(pHW20a-ZMO0976)

0.466 ± 0.005

6.95 ± 0.04

2.38 ± 0.05

1.41 ± 0.05

ZM4(pHW20a-ZMO1344)

0.396 ± 0.020

5.96 ± 0.94

2.18 ± 0.04

1.65 ± 0.21

ZM4(pHW20a-ZMO1673)

0.356 ± 0.013

5.44 ± 0.10

2.01 ± 0.04

1.16 ± 0.01

ZM4(pHW20a-ZMO1773)

0.306 ± 0.009

5.27 ± 0.45

1.78 ± 0.04

1.03 ± 0.00

ZM4(pHW20a-ZMO1984)

0.400 ± 0.002

5.20 ± 0.05

2.23 ± 0.03

1.55 ± 0.07

  1. In RM medium containing 2 g/L of furfural or 4 g/L of HMF at 30 °C. ZM4(pHW20a-gfp) is the control strain only expressing the reporter gene gfp. The fermentation performance was detected at the mid-log phase, where 12 h for furfural and 16 h for HMF. Standard deviations were derived from at least two independent determinations