Fig. 1From: Development of a genetically programed vanillin-sensing bacterium for high-throughput screening of lignin-degrading enzyme librariesSchematic diagram of the vanillin-sensing cell (VSC). The reporter plasmid construct comprises the vGFP gene cloned downstream of the vanillin-inducible promoter yeiW. The presence of vanillin results in a readily detectable fluorescence phenotype. For enzyme screening applications, any vanillin generated through extracellular degradation of lignin would activate the genetically encoded biosensorBack to article page