Fig. 3

a pH profile of recombinant cel12B, cel8C, and peh28 incubated for 1 h at 40 °C with 25 mg/ml CMC, for cel12B and cel8C, and 4 mg/ml polygalacturonic acid, for peh28. b Temperature profile of recombinant cel12B, cel8C, and peh28 incubated for 1 h at pH 5.4 with 25 mg/ml CMC for cel12B and cel8C, and 4 mg/ml polygalacturonic acid for peh28. One unit of enzyme activity was defined as the amount of enzyme releasing 1 µmol of reducing sugars per minute from the substrate under the assay conditions. Values presented are given as a mean of triplicates ± SE