Fig. 3

The scheme represents pyruvate dependent amino acid biosynthesis as well as engineered ethanol synthesis from pyruvate in Synechococcus 7002 expressing PDC and ADH. Metabolite data represent internal standard-corrected normalized responses, i.e. pool sizes in arbitrary units OD ⁻¹₇₅₀  mL⁻¹ of sample, from ethanol producer (filled circles) and WT (open circles). The black columns represent differential profiles, i.e. log₂-transformed ratios of producer over WT at each time point (Additional file 3). Data are averages of two producer and two WT cultures, respectively. Cells from each culture were harvested six times at each time point in the middle of a 12-h light phase (±10 min) as described under “Methods” section