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Table 1 Strains and plasmids used in this study

From: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance

Name Description Reference
E. coli
 JW513 BL21 containing pSKW01 (ApramycinR) This study
 JW514 BL21 containing pSKW02 (ApramycinR) This study
 JW515 BL21 containing pSKW04 (ApramycinR) This study
C. thermocellum
 LL1005 C. thermocellum DSM 1313 ΔpyrF (ura /5-FOAR) [25]
 JWCT02 LL1005 containing pDCW89 (ura +/5-FOAS) [25]
 JWCT06 LL1005 containing pSKW01 (ura +/5-FOAS) This study
 JWCT07 LL1005 containing pSKW02 (ura +/5-FOAS) This study
 JWCT08 LL1005 containing pSKW04 (ura +/5-FOAS) This study
Plasmids
 pDCW89 E. coli/C. thermocellum shuttle vector (ApramycinR) [13, 25]
 pDCW144 Intermediate vector 1 (ApramycinR) [15]
 pDCW148 Intermediate vector 2 (ApramycinR) This study
 pDCW171 Source of Teth39_1597 (bdhA) open reading frame (ApramycinR) [9]
 pSKW01 Expression vector containing PS-layer -bdhA (ApramycinR) This study
 pSKW02 Expression vector containing PClo1313_1809 -bdhA (ApramycinR) This study
 pSKW04 Expression vector containing Penolase -bdhA (ApramycinR) This study