Table 1 Strains and plasmids used in this study

E. coli

 JW513

BL21 containing pSKW01 (Apramycin^R)

This study

 JW514

BL21 containing pSKW02 (Apramycin^R)

This study

 JW515

BL21 containing pSKW04 (Apramycin^R)

This study

C. thermocellum

 LL1005

C. thermocellum DSM 1313 ΔpyrF (ura ⁻/5-FOA^R)

[25]

 JWCT02

LL1005 containing pDCW89 (ura ⁺/5-FOA^S)

[25]

 JWCT06

LL1005 containing pSKW01 (ura ⁺/5-FOA^S)

This study

 JWCT07

LL1005 containing pSKW02 (ura ⁺/5-FOA^S)

This study

 JWCT08

LL1005 containing pSKW04 (ura ⁺/5-FOA^S)

This study

Plasmids

 pDCW89

E. coli/C. thermocellum shuttle vector (Apramycin^R)

[13, 25]

 pDCW144

Intermediate vector 1 (Apramycin^R)

[15]

 pDCW148

Intermediate vector 2 (Apramycin^R)

This study

 pDCW171

Source of Teth39_1597 (bdhA) open reading frame (Apramycin^R)

[9]

 pSKW01

Expression vector containing P_S-layer -bdhA (Apramycin^R)

This study

 pSKW02

Expression vector containing P_{Clo1313_1809} -bdhA (Apramycin^R)

This study

 pSKW04

Expression vector containing P_enolase -bdhA (Apramycin^R)

This study