Fig. 2

Comparative saccharification potentials of PfCBH1 and TrCBH1 on polymeric cellulosic substrates. a, b The amount of total sugar obtained from the hydrolysis of microcrystalline cellulose (Avicel) using the purified GH7 CBH’s after 1- and 24-h incubation, respectively, while c and d show the hydrolysis potentials of optimized blends on ammonium hydroxide and sodium hydroxide pretreated wheat straws, respectively. In c, cellulase blend C1 contain—PfCBH1 to TrCBH1 ratio—[39:7], C2 is an inversion with—PfCBH1 to TrCBH1 ratio—[7:39]; C3 contains only PfCBH1 at 46%, while C4 contains only TrCBH1 at 46%. In d, cellulase blend D1 contain—PfCBH1 to TrCBH1 ratio [5:34]; D2 is an inversion with-PfCBH1 to TrCBH1 ratio—[34:5]; D3 contains only PfCBH1 at 39%; while D4 contains only TrCBH1 at 39%. All other components were kept as shown in (Additional file 1: Table S3). ****p < 0.0001, while ns: no significant difference at α = 0.05 using Tukey’s multiple comparison test. Error bars represent ±SE