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Table 1 Bacterial strains and plasmids used in this study

From: Citrobacter amalonaticus Y19 for constitutive expression of carbon monoxide-dependent hydrogen-production machinery

Strains and plasmids Genotype and description Source
Strains
 Y19 WT Citrobacter amalonaticus Y19; Ampr [7]
 Y19-PR1 Y19 with chromosomal replacement of native coo promoters with Pgap and Pnar* This study
 Y19-PR2 Y19-PR1 with UTR1(threefold) cooM This study
 Y19-PR3 Y19-PR1 with UTR1(6.5-fold) cooM This study
 Y19-PR1/pHyd-CO Y19-PR1 harboring pHyd-CO plasmid This study
 Y19-PR2/pHyd-CO Y19-PR2 harboring pHyd-CO plasmid This study
 Y19-PR3/pHyd-CO Y19-PR3 harboring pHyd-CO plasmid This study
 E. coli DH5 alpha Cloning host KCCM
Plasmids
 pUCPK/Pgap or Pnar* ColE1 ori, green florescent protein under the control of Pgap or Pnar* [23]
 pKOV/ΔPcoo::Pgap–nar* pKOV-pSC101-sacB; Cmr [24]
 pDK7 P15A ori; Cmr [8]
 pHyd-CO pDK7 p15A Pnar* cooKLXUH This study