Table 1 Bacterial strains and plasmids used in this study
Strains and plasmids | Genotype and description | Source |
|---|---|---|
Strains | ||
Y19 WT | Citrobacter amalonaticus Y19; Ampr | [7] |
Y19-PR1 | Y19 with chromosomal replacement of native coo promoters with Pgap and Pnar* | This study |
Y19-PR2 | Y19-PR1 with UTR1(threefold) cooM | This study |
Y19-PR3 | Y19-PR1 with UTR1(6.5-fold) cooM | This study |
Y19-PR1/pHyd-CO | Y19-PR1 harboring pHyd-CO plasmid | This study |
Y19-PR2/pHyd-CO | Y19-PR2 harboring pHyd-CO plasmid | This study |
Y19-PR3/pHyd-CO | Y19-PR3 harboring pHyd-CO plasmid | This study |
E. coli DH5 alpha | Cloning host | KCCM |
Plasmids | ||
pUCPK/Pgap or Pnar* | ColE1 ori, green florescent protein under the control of Pgap or Pnar* | [23] |
pKOV/ΔPcoo::Pgap–nar* | pKOV-pSC101-sacB; Cmr | [24] |
pDK7 | P15A ori; Cmr | [8] |
pHyd-CO | pDK7 p15A Pnar* cooKLXUH | This study |