|
Strains
|
|
Y19 WT
|
Citrobacter amalonaticus Y19; Ampr
|
[7]
|
|
Y19-PR1
|
Y19 with chromosomal replacement of native coo promoters with Pgap and Pnar*
|
This study
|
|
Y19-PR2
|
Y19-PR1 with UTR1(threefold) cooM
|
This study
|
|
Y19-PR3
|
Y19-PR1 with UTR1(6.5-fold) cooM
|
This study
|
|
Y19-PR1/pHyd-CO
|
Y19-PR1 harboring pHyd-CO plasmid
|
This study
|
|
Y19-PR2/pHyd-CO
|
Y19-PR2 harboring pHyd-CO plasmid
|
This study
|
|
Y19-PR3/pHyd-CO
|
Y19-PR3 harboring pHyd-CO plasmid
|
This study
|
|
E. coli DH5 alpha
|
Cloning host
|
KCCM
|
|
Plasmids
|
|
pUCPK/Pgap or Pnar*
|
ColE1 ori, green florescent protein under the control of Pgap or Pnar*
|
[23]
|
|
pKOV/ΔPcoo::Pgap–nar*
|
pKOV-pSC101-sacB; Cmr
|
[24]
|
|
pDK7
|
P15A ori; Cmr
|
[8]
|
|
pHyd-CO
|
pDK7 p15A Pnar* cooKLXUH
|
This study
|
