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Table 3 Various fatty acid compositions and contents of the transformants under heat shock conditions for 30 min ×3

From: Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga Chlamydomonas reinhardtii

Fatty acids

CC-849

aRP1.1

aRP1.2

aRP1.3

aRP1.4

aRP2.1

aRP2.2

aRP2.3

C12:0

0.046 ± 0.001

0.087 ± 0.001

0.074 ± 0.007

0.079 ± 0.002

0.08 ± 0.003

0.11 ± 0.031

0.084 ± 0.001

0.081 ± 0.001

C14:0

0.444 ± 0.016

0.625 ± 0.04

0.531 ± 0.047

0.556 ± 0.015

0.526 ± 0.011

0.69 ± 0.006

0.538 ± 0.007

0.523 ± 0.004

C16:0

20.208 ± 0.377

29.869 ± 0.812b

27.318 ± 2.346b

28.64 ± 0.535b

28.231 ± 0.61b

35.076 ± 1.781b

27.86 ± 0.999b

28.1 ± 0.558b

C16:1

3.865 ± 0.068

4.823 ± 0.826

4.98 ± 0.501b

4.68 ± 0.178b

4.114 ± 0.104b

4.335 ± 0.476

4.258 ± 0.128b

4.679 ± 0.108b

C18:0

3.257 ± 0.079

4 ± 0.177b

3.533 ± 0.156

4.014 ± 0.069b

3.84 ± 0.077b

5.094 ± 0.061b

3.27 ± 0.111

3.218 ± 0.054

C16:4

26.971 ± 0.282

36.832 ± 0.68b

34.999 ± 1.856b

38.697 ± 1.17b

35.366 ± 0.708b

33.142 ± 3.959a

36.463 ± 0.69b

38.57 ± 0.913b

C18:1 t

1.696 ± 0.177

4.201 ± 1.199b

2.764 ± 0.497b

2.22 ± 0.088b

1.872 ± 0.187

2.965 ± 0.32b

1.551 ± 0.119

1.857 ± 0.264

C18:2 t

5.687 ± 0.048

8.655 ± 1.497b

7.507 ± 0.558b

7.548 ± 0.165b

6.248 ± 0.065b

13.539 ± 0.682b

6.988 ± 0.43b

7.789 ± 0.208b

C18:3

8.042 ± 0.054

9.797 ± 0.304b

8.918 ± 0.353b

9.98 ± 0.229b

9.17 ± 0.472b

13.672 ± 1.253b

9.549 ± 0.318b

10.004 ± 0.31b

C18:3n3

34.281 ± 0.647

48.215 ± 2.613b

44.218 ± 2.514b

48.18 ± 1.527b

45.387 ± 1.151b

46.848 ± 5.121a

45.256 ± 0.94b

47.64 ± 1.178b

C20:1

1.793 ± 0.051

2.179 ± 0.348

1.751 ± 0.079

2.154 ± 0.05b

2.165 ± 0.089b

2.077 ± 0.276

2.099 ± 0.041b

2.161 ± 0.086b

C20:3

0.048 ± 0.001

0.097 ± 0.003b

0.089 ± 0.01b

0.093 ± 0.001b

0.097 ± 0.005b

0.125 ± 0.05a

0.111 ± 0.034a

0.095 ± 0.005b

C20:4

0.063 ± 0.001

0.124 ± 0.019b

0.104 ± 0.006b

0.11 ± 0.004b

0.107 ± 0.001b

0.145 ± 0.046b

0.105 ± 0.002b

0.105 ± 0.003b

C22:2

0.041 ± 0.002

0.091 ± 0.009b

0.078 ± 0.009b

0.081 ± 0.002b

0.083 ± 0.002b

0.111 ± 0.046b

0.084 ± 0.002b

0.081 ± 0.003b

C24:0

0.266 ± 0.012

0.455 ± 0.006b

0.4 ± 0.027b

0.415 ± 0.012b

0.415 ± 0.005b

0.532 ± 0.11b

0.389 ± 0.009b

0.386 ± 0.002b

C22:6

0.073 ± 0.004

0.14 ± 0.001b

0.142 ± 0.023b

0.144 ± 0.003b

0.144 ± 0.003b

0.188 ± 0.085a

0.142 ± 0.002b

0.147 ± 0.004b

TFA

106.78 ± 12.24

150.19 ± 2.882b

137.406 ± 8.66b

140.212 ± 9.72b

137.845 ± 4.65b

158.65 ± 18.42b

138.743 ± 8.4b

145.44 ± 11.61b

  1. For applying heat shock (HS), 400 mL cells (WT and transgenic algae) in mid-logarithmic phase were incubated at 40 °C and light intensity 20 μmol/m2/s for 30 min. As for triple heat shocks, a 4-h recovery period was utilized between each heat shock
  2. The data represent the means ± SD of three replicate experiments and were analyzed by Student’s t-test (n = 3)
  3. aP <0.05; bP <0.01. aRP1.1, aRP1.2, aRP1.3, aRP1.4 is transformed amicroRNA-PEPC1 into C. reinhardtii; aRP2.1, aRP2.2, aRP2.3 is transformed amicroRNA-PEPC2 into C. reinhardtii