Moderate alkali-thermophilic ethanologenesis by locally isolated Bacillus licheniformis from Pakistan employing sugarcane bagasse: a comparative aspect of aseptic and non-aseptic fermentations

Table 1 Ethanol yield of Bacillus licheniformis KU886221 using differently pretreated SCB

Serial no.	Pretreatment	Incubation (h)	Total sugars (g/L)^a	Ethanol (g/L)
1	2% H₂SO₄	0	41.227^b ± 0.430	0ⁱ
		24	36.556^cd ± 0.675	0.926^gh ± 0.229
		72	32.943^efg ± 1.009	2.103^cde ± 0.167
		120	31.434^fgh ± 0.570	2.61^abc ± 0.118
2	3% H₃PO₄	0	39.573^de ± 0.718	0ⁱ
		24	35.720^gh ± 0.325	1.126^fgh ± 0.117
		72	31.277^hi ± 0.657	2.44^bcd ± 0.092
		120	29.997ⁱ ± 0.928	2.863^ab ± 0.154
3	1% HCl	0	44.964^a ± 0.527	0ⁱ
		24	39.427^bc ± 0.562	1.155^fgh ± 0.159
		72	34.533^def ± 0.595	2.06d^e ± 0.097
		120	32.317^fg ± 0.202	2.56^bcd ± 0.066
4	1 N NaOH	0	45.919^a ± 0.239	0ⁱ
		24	41.371^b ± 0.523	1.24^fgh ± 0.244
		72	35.687^de ± 0.603	2.793^ab ± 0.164
		120	31.800^fg ± 0.443	3.313^a ± 0.266
5	Control (untreated)	0	35.218^de ± 0.248	0ⁱ
		24	31.053^gh ± 0.335	0.69^h ± 0.039
		72	26.943ⁱ ± 0.329	1.553^efg ± 0.073
		120	23.996^j ± 0.305	1.68^ef ± 0.037

^aGlucose + xylose. Values signify mean ± SE of triplicates. Values that do not share an alphabet are significantly different from each other. Multi-factor analysis of variance (P < 0.05)

ISSN: 2731-3654