Fig. 1

Summary of modifications in the iCth446 GSM model after updating it from the previous iSR432 reconstruction. Updated reactions in iCth446: The cofactors highlighted in green (in reactions HEX1, PFK, PGK, and ME) were added in the model and those in red (for reactions HEX1, PFK, and ME) were removed. In addition, reactions ME and PEPCK were made reversible. Dashed lines in gray indicate an example of thermodynamically infeasible cycle of three reactions (ODC, MDH, and ME). The cycle was resolved by removing ODC. NFN was added to the model to allow electron transfer between reducing equivalents. The values alongside the reactions are their FBA-predicted fluxes (in mmol/gDW/h) consistent with the wild-type experimental cellobiose uptake and growth rates [65]