Fig. 5

Cross-validation analysis of k-ctherm118 model. The gray bars represent the average scaled deviation of the predicted steady-state fluxes upon cross-validation of the training dataset. The first 19 datasets represent mutants with experientially measured fermentation yields, the penultimate dataset represents Δgldh mutant with measured intracellular concentrations, and the final dataset represents the experimentally measured Michaelis–Menten constants. The white bars correspond to the average scaled deviation of the predicted steady-state flux distribution from the experimental measurements while including all datasets. The difference between two bars represents the reduction in the accuracy of the model predictions upon excluding the flux dataset of the corresponding mutant