Fig. 6

Impact of limiting nitrogen source in the media on C. thermocellum metabolism. a Change in pathway fluxes and metabolite concentration on reducing ammonium uptake activity to 20% of the wild-type activity. The numbers represent the flux values (mol/mol cellobiose) normalized to cellobiose uptake (except for those in parentheses represent concentration change) for the wild-type (gray color) to ammonia-limited (green or red color representing upregulation or downregulation, respectively) conditions. All fluxes were normalized to cellobiose uptake. b Comparison of model-predicted yield change with the experimentally measured values