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Table 3 Experimentally measured kinetic parameters (data ± standard deviation) (R Sparling, unpublished data; [42])

From: Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations

Enzyme

Substrate

K m [mM]

k cat [1/s]

PGI

d-Glucose 6-phosphate

1.92 ± 0.57

1637.50 ± 798.25

PGMT

alpha-d-Glucose 1-phosphate

0.41 ± 0.04

190 ± 19

HK

GTP

0.43 ± 0.04

 

PFK

ppi

0.23 ± 0.02

30.14 ± 3.01

PGK

gtp

0.25 ± 0.02

 

RPI

Ribose-5-phosphate

17 ± 1.7

51998 ± 5199.8