Table 5 Parameters selected for analysis in study 1
Separation stage and task | Parameter | Base case (nominal) | Range | References |
|---|---|---|---|---|
Stage I: input parameter | Biomass titer in input process stream | 5 g/L | (0.1–200) | |
Stage I: task—cell harvesting | Cell separation efficiency for centrifugation (Cnt,1) | 80% | (70–95) | |
Cell retention factor for filtration (Ftt,1) | 80% | (70–95) | [85] | |
Stage I: task—cell disruption | % Release for acid hydrolysis (Ahy) | 85% | (80–98) | [122] |
% Release for bead mill (Bml) | 85% | (80–98) | [123] | |
% Release for enzyme lysis (Ely) | 90% | (85–98) | [124] | |
Stage I: task—phase isolation | Amount of agent required for differential digestion (Ddg) | 0.4 kg/kg other cell components | (0.05–3) | [109] |
Amount of solvent required for solubilization (Slb) | 0.4 kg/kg product | (0.05–3) | [107] |