Table 2 Effects of metal ions and NaCl on the enzymatic activities of recombinant enzymes (each treatment had three biological replicates) aSubstrate: refer to Fig. 5a
Enzymes (product of:) | Substratea | No additive | Relative activities (%) | |||||
|---|---|---|---|---|---|---|---|---|
MgCl2 | MnCl2 | NaCl | ||||||
5 mM | 20 mM | 5 mM | 20 mM | 200 mM | 2000 mM | |||
Gene 1 | pNP-Gal | 100 ± 1.8 | 113.1 ± 9.1 | 114.1 ± 9.6 | 97.7 ± 6.5 | 95.5 ± 9.2 | 91.7 ± 3.5 | 90.9 ± 3.6 |
Gene 2 | pNP-Xyl | 100 ± 4.9 | 124.5 ± 0.7 | 129.9 ± 4.9 | 108.6 ± 4.4 | 105.0 ± 3.2 | 112.4 ± 12.4 | 118.6 ± 9.3 |
Gene 2 | pNP-Ara | 100 ± 17.6 | 150.5 ± 19.4 | 106.4 ± 26.0 | 135.5 ± 7.6 | 81.6 ± 1.2 | 183.9 ± 53.1 | 75.1 ± 1.9 |
Gene 5 | pNP-Glu | 100 ± 2.7 | 85.4 ± 3.4 | 94.0 ± 6.3 | 95.6 ± 2.3 | 88.7 ± 1.0 | 102.7 ± 3.8 | 59.9 ± 2.4 |
Gene 6 | pNP-Glu | 100 ± 4.1 | 105.5 ± 1.4 | 112.8 ± 5.5 | 92.8 ± 2.3 | 111.1 ± 1.0 | 159.5 ± 3.6 | 119.3 ± 5.1 |