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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

Fig. 3

Activity of MtLPMO9B towards amorphous cellulose in the presence and absence of MtPPO7. HPAEC elution pattern of regenerated amorphous cellulose (RAC; 1.5 mg mL−1) incubated with MtLPMO9B (5.0 μg mL−1) with (yellow) and without (red) MtPPO7 (5.0 μg mL−1) addition in the presence of a guaiacol (no. 9 specified in Table 1, 2 mM) and b 3-methylcatechol (no. 17 specified in Table 1, 2 mM) after 24 h. The incubation of RAC with MtLPMO9B results in the formation of non-oxidized gluco-oligosaccharides (GlcOSn) and C1-oxidized gluco-oligosaccharides (GlcOS n # ). Based on HPAEC, integrated peak areas are shown as the total sum of released non-oxidized (shaded red and yellow) and C1-oxidized (red and yellow) gluco-oligosaccharides after incubation of RAC (1.5 mg mL−1) with MtLPMO9B only (red bars, 5.0 μg mL−1) and MtLPMO9B together with MtPPO7 (yellow bars, 5.0 μg mL−1) in the presence of c guaiacol and d 3-methylcatechol. All incubations were performed in duplicate, and the standard deviations are presented as error bars. See “Methods” for data analysis and details

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