Table 1 Bacterial strains used in this study
From: The significance of proline and glutamate on butanol chaotropic stress in Bacillus subtilis 168
Strains | Relevant genotype and characteristic(s) | Source | Remarka |
|---|---|---|---|
168 | A wild-type Bacillus subtilis (trpC2) | BGSCb | BGSC 1A1 |
146 | 168pro(AB)− A proline auxotroph strain with γ-glutamyl phosphate reductase gene (proA) and γ-glutamyl kinase gene (proB) disruption | BGSCb | BGSC 1A652 |
GP16 | 168∆(gltP::cam r) A strain with glutamate transporter gene disruption | This study | BMGC 162 |
B934 | 168∆(proB::spc r) A proline auxotroph strain with γ-glutamyl kinase gene disruption | This study | BMGC 163 |
BH901 | 168∆(proB::spc r) ∆(proHJ::cam r) or B934∆(proHJ::cam r) A proline auxotroph strain with proB, ∆1-pyrroline-5-carboxylate reductase gene (proH) and a proB-like γ-glutamyl kinase gene (proJ) disruption | This study | BMGC 164 |
H972 | 168∆(proHJ::cam r) A proline auxotroph strain with ∆1-pyrroline-5-carboxylate reductase gene (proH) and a proB-like γ-glutamyl kinase gene (proJ) disruption | This study | BMGC 161 |
HK | 168 carrying pHK vector | This study | BMGC 165 |
HK-GPOX | 168 carrying pHK-gltPOX A recombinant strain with an overexpressed GltP under P43 promoter | This study | BMGC 167 |
HK′-HJOX | 168 carrying pHK-HJOX A recombinant strain with an overexpressed ProHJ under its original promoter | This study | BMGC 166 |