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Table 1 Bacterial strains used in this study

From: The significance of proline and glutamate on butanol chaotropic stress in Bacillus subtilis 168

Strains Relevant genotype and characteristic(s) Source Remarka
168 A wild-type Bacillus subtilis (trpC2) BGSCb BGSC 1A1
146 168pro(AB)
A proline auxotroph strain with γ-glutamyl phosphate reductase gene (proA) and γ-glutamyl kinase gene (proB) disruption
BGSCb BGSC 1A652
GP16 168∆(gltP::cam r)
A strain with glutamate transporter gene disruption
This study BMGC 162
B934 168∆(proB::spc r)
A proline auxotroph strain with γ-glutamyl kinase gene disruption
This study BMGC 163
BH901 168∆(proB::spc r) ∆(proHJ::cam r) or B934∆(proHJ::cam r)
A proline auxotroph strain with proB, ∆1-pyrroline-5-carboxylate reductase gene (proH) and a proB-like γ-glutamyl kinase gene (proJ) disruption
This study BMGC 164
H972 168∆(proHJ::cam r)
A proline auxotroph strain with ∆1-pyrroline-5-carboxylate reductase gene (proH) and a proB-like γ-glutamyl kinase gene (proJ) disruption
This study BMGC 161
HK 168 carrying pHK vector This study BMGC 165
HK-GPOX 168 carrying pHK-gltPOX
A recombinant strain with an overexpressed GltP under P43 promoter
This study BMGC 167
HK′-HJOX 168 carrying pHK-HJOX
A recombinant strain with an overexpressed ProHJ under its original promoter
This study BMGC 166
  1. cam r chloramphenicol-resistant gene, spc r spectinomycin-resistant gene
  2. aThe recombinant strains generated in this study were deposited at BIOTEC Culture Collection, National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand (shown as BMGC number)
  3. bBGSC: Bacillus Genetic Stock Center (Columbus, OH, USA)