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Table 2 Upregulated protein spots identified as carbohydrate-active enzymes

From: Metaproteomics-guided selection of targeted enzymes for bioprospecting of mixed microbial communities

Spot no. Method No. of peptides Of which unique Score (−10lgP) Database entry by PEAKS DB and SPIDER Name Categorya
1 PEAKS DB
SPIDER
8 2 216.7 Gi|489608295b Cellulose 1,4-β-cellobiosidase CAZy GH 9, NC, SP (1–27)
9 3 246.3
2 PEAKS DB
SPIDER
8 2 162.1 Gi|489608295b Cellulose 1,4-β-cellobiosidase CAZy GH 9, NC, SP (1–27)
11 4 172.5
3 PEAKS DB
SPIDER
5 2 109.7 Gi|489608295b
Gi|740458809c
Cellulose 1,4-β-cellobiosidase CAZy GH 9, NC, SP (1–27)
13 8 148.5
4 PEAKS DB
SPIDER
4 2 108.6 Gi|489608295b
Gi|740458809c
Cellulose 1,4-β-cellobiosidase CAZy GH 9, NC, SP (1–27)
4 2 116.6
5 PEAKS DB
SPIDER
1 1 91.99 Gi|489608295b Cellulose 1,4-β-cellobiosidase CAZy GH 9, NC, SP (1–27)
1 1 91.99
6 PEAKS DB
SPIDER
7 5 126.8 Gi|489614249b Cellulose 1,4-β-cellobiosidase CAZy GH 9, NC, SP (1–27)
8 3 133.8
7 PEAKS DB
SPIDER
17 4 216.7 Gi|575088796c Endo 1-4-β-xylanase CAZy GH 11, NC, SP (1–29)
34 14 246.3
8 PEAKS DB
SPIDER
4 2 121.9 Gi|489614855b
Gi|489609419c
Carbohydrate-binding protein
Endo 1-4-β-xylanase precursor
CAZy GH 11, NC, SP (1–29)
4 4 121.9
9 PEAKS DB
SPIDER
5 3 97.4 Gi|489614249b
Gi|575088796c
Cellulose 1,4-β-cellobiosidase
Endo 1-4-β xylanase
CAZy GH 9, NC, SP (1–27)
CAZy GH 11, NC, SP (1–29)
6 3 106.1
10 PEAKS DB
SPIDER
8 2 149.6 Gi|575088170c Cellobiose phosphorylase CAZy GH 11, TM, SP (1–27)
9 3 152.6
11 PEAKS DB
SPIDER
9 2 136.7 Gi|575088170c
Gi|489613736b
Cellobiose phosphorylase
Glycosyl transferase
CAZy GH 94, TM
9 2 139.2
12 PEAKS DB
SPIDER
2 1 74.76 Gi|740457862c Glycosyl transferase CAZy GH 94, TM
2 1 74.76
13 PEAKS DB
SPIDER
7 2 120.6 Gi|489616357b β-Glucosidase CAZy GH 1, TM (weak), NC
9 2 124.2
14 PEAKS DB
SPIDER
14 4 243.8 Gi|553726407b
Gi|489608943b
Hypothetical Ig domain protein Carbohydrate act. enzyme domain, NC, SP (1–26)
22 10 260.9
15 PEAKS DB
SPIDER
8 4 178.1 Gi|553726407b
Gi|489608943b
Hypothetical Ig domain protein Carbohydrate act. enzyme domain, NC, SP (1–26)
12 7 193.2
16 PEAKS DB
SPIDER
9 3 166.4 Gi|553726407b
Gi|489608943b
Hypothetical Ig domain protein Carbohydrate act. enzyme domain, NC, SP (1–26)
15 5 184.8
17 PEAKS DB
SPIDER
4 4 155.9 Gi:489613676b Hypothetical Ig domain protein Carbohydrate act. enzyme domain, NC, SP (1–26)
4 4 155.9
  1. aIn CAZy database; GH glycoside hydrolase family no., NC non-cellulosomal, SP signal peptide, signal peptide sequence length in brackets, TM transmembrane
  2. b Ruminiclostridium thermocellum
  3. c Clostridium straminisolvens