Fig. 2

Transcript abundance of the GH family genes in B. sorokiniana. A standard curve using known amounts of cDNA was prepared for the three homologs of BsGH6, six belonging to BsGH7 and two of BsGH45; (further details in “Results”) in four replicates. Based on the standard curve, cDNA corresponding to 150 ng of total RNA was used to evaluate the absolute transcript amount based on the respective CT values. Three independent experiments were conducted, each comprising four replicates, and the mean values were used to plot the graph. Data are represented as mean ± SE. Assistat 7.6 beta was used for statistical analysis (DMRT-Duncan multiple range test). The Duncan test at a level of 5% of probability was applied. Bars with the same letter do not differ statistically between themselves (p ≤ 0.05)