The advanced strategy for enhancing biobutanol production and high-efficient product recovery with reduced wastewater generation

Table 3 The effects of washing times on ABE fermentation and wastewater generation

	(0-W)/HN	(1-W)/HN	(2-W)/HN	(3-W)/HN	8-W
Initial pH	13.1	13.1	13.1	13.1	13.1
Washed/HCl-neutralized pH	13.1/8.8	11.9/8.8	10.7/8.8	9.7/8.8	8.8/8.8
Initial JAS, g	200	200	200	200	200
Pretreated JAS, g	172.7	170.1	165.6	162.3	148.6
Initial glucose, g/L	33.0	36.5	38.8	46.3	48.2
Initial xylose, g/L	12.3	12.5	12.4	13.9	13.6
Butanol, g/L	8.6	9.7	10.1	10.8	11.2
Acetone, g/L	4.8	4.9	5.2	5.7	5.8
Ethanol, g/L	0.2	0.2	0.2	0.2	0.2
Butanol yield, mg/g-JAS	29.7	33.0	33.5	35.5	33.5
ABE yield, mg/g-JAS	47.0	50.3	51.3	54.2	51.1
Water volume, L	0	3	6	9	24
Water consumption, L/g-butanol	0	0.45	0.90	1.3	3.6

(0-W)/HN unwashed/HCl-neutralization, (1-W)/HN washing 1 time/HCl-neutralization, (2-W)/HN washing 2 times/HCl-neutralization, (3-W)/HN washing 3 times/HCl-neutralization, 8-W washing 8 times

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