Fig. 9

Lipid accumulation of Chlamydomonas reinhardtii strain CC4533 and its knock-out mutants. Lipid accumulation of Chlamydomonas reinhardtii strain CC4533 (1) and its knock-out mutants deficient in glutamate synthase/NADH-dependent (2), aspartate aminotransferase (3), glutamine synthetase (4), alanine aminotransferase (5), glutamate synthase/Fd-dependent (6), pyruvate kinase (7), and citrate synthase (8) cells labeled in vivo with Bodipy 505/515 were analyzed by FCM (A, B) and CLSM (C) after 0 h (a), 12 h (b), 24 h (c), and 48 h (d) of N starvation. A, B The control (0 h of sample 1) value of lipid content was set to 1 for easy comparison; the significance of the differences between the CC4533 (1) and other mutants in each time point was tested using a one-way ANOVA. *p < 0.05. C CLSM images of cells with Bodipy 505/515 fluorescence (green) in each treatment were recorded, all of the figures are representative of three replicated studies with similar findings, and the size of the scale bar is shown directly on the image