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Table 4 Characterization of purified GH6 enzymes

From: Management of enzyme diversity in high-performance cellulolytic cocktails

Enzyme

Mol. mass (kDa)

CBM presence

Optimal temperature & pH

Thermostabilitya

Azo-CMC (U/g)

Azo-WAX (U/g)

Avicel (U/g)

G2Q998 (EG VI)

47

No

65 °C; 5.5–6.0

Unstable

5492.1

1335.6

89.7

G2QFW6 (CBH IIa)

43

No

60 °C; 4.5

Stable

431.5

207.2

102.5

G2QA39 (CBH IIb)

70

Yes

60 °C; 5.0

Stable

560.0

108.0

192.6

  1. aStable/unstable means presence/absence of the protein band in SDS-PAGE after incubation at pH 5.0 and 50 °C during 24 h