Fig. 2
From: A simple method to control glycolytic flux for the design of an optimal cell factory
Redesign of the 5′-UTR for ptsG based on base-pairs between SgrS and ptsG. The asterisks indicate the predicted base-pairing region of SgrS required for the translational repression of ptsG mRNA [24]. In particular, the minimal base-pairs for SgrS action for effective translational inhibition are shown in bold [26, 48]. The italic letters represent the Shine–Dalgarno (SD) sequence and the initiation codon for ptsG. The changed nucleotides with respect to the wild-type sequence of the ptsG UTR are underlined