Fig. 5
From: Metabolic engineering of Escherichia coli for production of n-butanol from crude glycerol
Microaerobic production of n-butanol in the strain with the suppressed TCA cycle. Escherichia coli strain BuT-16 was engineered by suppression of the gltA gene. The strain was grown in M9Y medium containing either 20 g/L pure glycerol or crude glycerol. The fermentations using pure glycerol and crude glycerol were carried out for 24 and 40 h, respectively. The experiments were conducted in triplicate. Keys a the fermentation with pure glycerol; b the fermentation with crude glycerol