Fig. 2
Functional characterization of NoDGAT1s in Saccharomyces cerevisiae cells. a TLC analysis of lipids extracted from TAG-deficient S. cerevisiae H1246 cells transformed with NoDGAT1s. Empty vector (EV) and ScDGA1 were used as the negative and positive controls, respectively. b BODIPY staining of NoDGAT1-expressing H1244 cells. Left, bright field; right, fluorescent field. Green fluorescence indicates the BODIPY-bound lipid droplets. c Growth of NoDGAT1-expressing H1244 cells (OD600, 1) in different dilutions (10- to 1000-fold) plated on 0.25 mM C18:1-containing solid medium for 3 days. 5 µL cells were plated. d Growth of NoDGAT1-expressing H1244 cells as affected by the feeding of free fatty acids of C16:1, C18:1, C18:2, and C18:3 after a 12-h cultivation. The concentration of FFAs was 125 µM. e TLC analysis of lipids extracted from S. cerevisiae INVSc1 cells transformed NoDGAT1s. f Relative abundance of fatty acids in TAG from S. cerevisiae INVSc1 cells transformed NoDGAT1s. Data in (d) and (f) are expressed as mean ± SD (n = 3)