Fig. 6

NoDGAT1A knockdown attenuated TAG accumulation and altered its fatty acid composition. a NoDGAT1A mRNA levels in NoDGAT1A knockdown lines and the empty vector (EV) control, as determined by quantitative real-time PCR. b TAG contents in NoDGAT1A knockdown lines and EV. c, d Relative abundance of fatty acids in the sn-1/sn-3 (c) and sn-2 (d) positions of TAG in NoDGAT1A knockdown lines and EV under nitrogen-depleted conditions (24 h). Data in (a–d) are expressed as mean ± SD (n = 3). Algal cells grown in nitrogen-replete medium for 4 days (considered as 0 h of nitrogen depletion) were used for nitrogen-depletion experiment. Asterisks indicate the significant difference compared with EV (t-test, P < 0.05)