Fig. 2

Enzymatic hydrolysis glucose yields of untreated and alkaline pretreatment of anatomical fractions. Enzymatic hydrolysis of untreated biomass was performed on AIR + de-starched biomass, while alkaline pretreatment was performed using 10% w/w NaOH loading at 80 °C for 1 h. Glucose hydrolysis yields are presented as the percent of total cell wall glucan released as monomeric glucose. Enzyme loading was 30 mg protein/g glucan for untreated fractions and 10 mg protein/g glucan for alkaline pretreated fractions with a ratio of 2:1 CTec3 to HTec3. Replicates (n = 3) are displayed as averages with standard deviations