Fig. 1

Concept of the synthetic co-culture of S. elongatus cscB and P. putida cscAB for the production of PHA from CO₂ and light. CO₂ is fixed via the Calvin cycle to make sucrose, which in turn is secreted into the surrounding medium by the activity of the heterologous sucrose permease CscB. CscA produced by P. putida cscAB leaks out of the cell, where it splits sucrose extracellularly [16]. The monomers (glucose and fructose) are metabolized by P. putida cscAB, and polyhydroxyalkanoates (PHA) are accumulated in the cytoplasm [15]