Fig. 5

Quantitative PCR analysis of gene expression levels. The expression levels of xyn1 (GH11), xyn2 (GH11), xyn5 (GH11), xyn3 (GH10), and xyn4 (GH30) in RUT-C30, Δsxlr, Osxlr, and Rsxlr when wheat bran and Avicel (a) and xylan (b) were used as carbon sources, expression levels were normalized to the signal of rpl6e. RNA was extracted after 4, 8, and 12 h. Error bars represent the standard deviation of three biological replicates. c Expression levels of sxlr in RUT-C30, the xyr1 deletion mutant (Δxyr1), and the xyr1-overexpression strain (Oxyr1); and xyr1 expression levels in RUT-C30, Δsxlr, and Osxlr when wheat bran and Avicel were used as the carbon source. Expression levels were normalized to the signal of β-actin. RNA was extracted after 4, 8, and 12 h. Error bars represent the standard deviation of three biological replicates