Fig. 9

Xylose fermentation by recombinant yeast strains expressing single amino acid substitution mutants of RsXI-C1. Xylose consumed by S. cerevisiae cells expressing the indicated RsXI-C1 point mutants, obtained by site-saturation mutagenesis of Asn-337, after 72-h fermentation under microaerobic condition. Xylose fermentation was carried out as described in "Methods". In all cases, initial cell density (absorbance at 600 nm, OD₆₀₀) was 1.0. Error bars indicate standard deviations of biological duplicates or triplicates